Synthetic Cationic Amphiphile for Liposome-Mediated DNA Transfection with Less Cytotoxicity.
نویسندگان
چکیده
منابع مشابه
Cationic liposome-mediated RNA transfection.
We have developed an efficient and reproducible method for RNA transfection, using a synthetic cationic lipid, N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA), incorporated into a liposome (lipofectin). Transfection of 10 ng to 5 micrograms of Photinus pyralis luciferase mRNA synthesized in vitro into NIH 3T3 mouse cells yields a linear response of luciferase activity. The...
متن کاملpreparation and in vitro transfection of modified cationic liposome-linear polyethyleneimine-dna nanocomplexes for gene delivery
متن کامل
Cholesterol enhances cationic liposome-mediated DNA transfection of human respiratory epithelial cells.
Cationic liposome transfection reagents are useful for transferring polynucleotides into cells, and have been proposed for human pulmonary gene therapy. The effect of adding cholesterol to cationic lipid preparations has been tested by first formulating the cationic lipid N-[1-(2,3-dioleoyloxy)propyl-N-[1-(2-hydroxy)ethyl]-N,N-dimethyl ammonium iodide (DORI) with varying amounts of dioleoylphos...
متن کاملHerpes simplex virus-enhanced cationic lipid/DNA-mediated transfection.
Liposome plasmid DNA complexes (lipoplexes) are often inefficient in mediating gene transfer and expression because of DNA degradation in lysosomal vesicles. Because herpes simplex virus (HSV) enters cells by fusion of the virus envelope with the plasma membranes, thereby overriding the endosomal pathway, HSV/lipoplex mixtures could be useful for improving gene transfer particularly when the mi...
متن کاملNucleoside phosphocholine amphiphile for in vitro DNA transfection.
A new transfection reagent based on nucleoside phosphocholine amphiphile leading to high transfection efficacy and low cytotoxicity is described. TEM, ethidium bromide displacement assays, agarose gel electrophoresis and SAXS studies support the formation of lipoplexes for the transfection of CHO cells.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biological and Pharmaceutical Bulletin
سال: 1996
ISSN: 0918-6158,1347-5215
DOI: 10.1248/bpb.19.860